In order to explore the effect of Atractylodes macrocephala（AM）on humoral immunity in chickens, three kinds of extract of AM were prepared. Astragalus polysaccharides (APS) was used for positive control, the different extracts were added into the feedstuff of chickens. The levels of Newcastle disease antibody in chickens of each group were respectively detected by ELISA after 8 days and 15 days continuous administration. The results showed that, after continuous administration of 8 days, compared with the blank group, the differences among groups were not significant. The high dose of AM ethanol extract and APS increased the antibody level of Newcastle disease by 15.35% (P>0.05) and 15.32% (P>0.05) respectively. After continuous administration of 15 days, compared with the blank group, the level of antibody in each group increased in varying degrees. High dose AM aqueous extract and low dose AM ethanol extract increased the antibody level by 61.37% (P<0.05) and 32.57% (P<0.05) respectively. Compared with the positive group, the high dose of AM aqueous extract increased the antibody level by 45.02% (P< 0.05). The high dose of AM aqueous extract was continuous administrated for 15d had the best effect on enhancing the humoral immunity of chickens.