In this paper, the response surface methodology was used to optimize the enzymatic extraction of Polysaccharides from fruiting body of Auricularia polytricha. In order to extract the polysaccharides from fruiting body of Auricularia polytricha by Cellulase, the optimum process parameters of 4 main factors, such as the amount of enzyme, the pH value, the extraction temperature and the extraction time, were established on the basis of the response surface test through the single factor test. The results showed that the optimum technological conditions were as follows: the dosage of cellulase was 3 774 U/g, pH=4.9, the extraction temperature was 50.2℃, and the extraction time was 135min. Under this condition, the extraction rate of polysaccharide was 15.47%, and the relative error of the model predicted value 15.52% was only 0.323%.