Pomegranate wilt is a soil-borne disease caused by Ceratocystis fimbriata Ellis. et Halsted, which has caused serious losses to the pomegranate industry.Using antagonistic microorganisms to inhibit the reproduction of pathogenic bacteria and reduce their harm has become a research hotspot in the field of pomegranate wilt prevention and control.This article will screen strains with antagonistic effects on the pathogen of pomegranate wilt and study their inhibitory effects, in order to provide effective biological control resources to control pomegranate wilt.Fungi isolated from the rhizosphere soil of pomegranates were screened by measuring spore production, growth rate, antagonistic coefficient, and plate confrontation.At the same time, these biocontrol strains were identified by morphological and molecular biological techniques.Finally, the control effect of the strain on pomegranate wilt was evaluated through pot experiments.Trichoderma harzianum was identified as an effective strain for inhibiting the growth of the pathogen of pomegranate wilt, and the control effect of pot cultivation was up to 79.63%. This strain showed significant biological control effect on pomegranate wilt and had potential for application value.