基于色泽与多指标成分分析的川明参质量快速评价研究
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四川省中医药科学院,四川 成都 610041

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四川省科技厅重点研发项目(2022YFS0592)。


Study on Rapid Quality Assessment of Chuanminshen violaceum Sheh et Shan Based on Color and Multi-Index Component Determination
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Sichuan Academy of Chinese Medicine Sciences, Chengdu, Sichuan 610041, China

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    摘要:

    目的 探索川明参外观色泽与多个指标成分间的关系,为快速、高效筛选优质川明参药材提供参考。方法 以不同商品规格川明参药材为研究对象,首先利用分光测色仪测定川明参粉末色度值,然后采用热浸法、硫酸-苯酚法以及超高效液相色谱法分别测定水溶性浸出物、醇溶性浸出物、多糖、欧前胡素等指标成分质量分数,最后使用IBM SPSS Statistics 26.0软件分析色度值与各指标成分间的相关性。结果 川明参粉末色度值L*与醇溶性浸出物、水溶性浸出物以及欧前胡素质量分数呈极显著负相关(P<0.01),与多糖质量分数呈显著负相关(P<0.05);ΔE*与水溶性浸出物及欧前胡素质量分数呈极显著负相关(P<0.01),与醇溶性浸出物、多糖质量分数呈显著负相关(P<0.05);a*b*与醇溶性浸出物、水溶性浸出物、欧前胡素质量分数呈极显著正相关(P<0.01),与多糖质量分数呈显著正相关。结论 色度值可作为快速判断评价川明参药材质量的指标,为全面评价川明参药材品质提供科学依据。

    Abstract:

    Objective Explored the correlation between color and several index components of Chuanminshen violaceum Sheh et Shan, aiming at providing a reference for rapid and efficient screening of high quality Chuanminshen violaceum.Method Spectrophotometer was used to determine the color value of Chuanminshen violaceum; hot leaching method, sulfuric acid-phenol method, ultra-high performance liquid chromatography method were used to determine water-soluble extract, alcohol-soluble extract, polysaccharide and imperatorin respectively. IBM SPSS Statistics 26.0 was used to analyze the correlation between color and index component.Result The value of L* had extremely significant negative-correlation with alcohol-soluble extract, water-soluble extract and imperatorin (P<0.01), and significant negative-correlation with polysaccharide (P<0.05). The value of ΔE* had extremely significant negative correlation with water-soluble extract and imperatorin (P<0.01), and significant negative correlation with alcohol-soluble extract and polysaccharide (P<0.05). However, the values of a* and b* had extremely significant positive correlation with alcohol-soluble extract, water-soluble extract and imperatorin (P<0.01), and significant positive correlation with polysaccharide (P<0.05).Conclusion The color values can be used as the index for rapid identification of quality of Chuanminshen violaceum, which provided scientific basis for comprehensive assessment of the quality of Chuanminshen violaceum.

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向缅,胡平,张美,林娟,周霞,杨玉霞,周先建.基于色泽与多指标成分分析的川明参质量快速评价研究[J].西昌学院学报(自然科学版),2023,37(4):6-13.

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  • 收稿日期:2023-09-27
  • 最后修改日期:2023-10-20
  • 录用日期:2023-11-14
  • 在线发布日期: 2024-02-22