Acid producing bacteria are a kind of important bacteria and microorganisms in high-temperature Daqu. Their main role is to provide flavor components for liquor and increase liquor flavor through fermentation in the process of liquor brewing. However， there are few studies on the characteristics of acid producing bacteria in high-temperature Daqu. In this study， 7 strains were obtained by preliminary screening through enrichment culture and calcium plate from High-temperature Daqu. Through qualitative and quantitative experiments， it was found that RS-6 strain had the highest acid production， reaching 2.13 g/L. The strain RS-6 was further studied by ethanol resistance， acetic acid resistance， salt resistance and high temperature resistance experiments. It was found that the strain had the quality of 7% ethanol resistance， 3% acetic acid resistance， 0.8% salt resistance and its optimum growth temperature was 30 ℃. The study indicated that a strain of acid producing bacteria with high acid production， high ethanol tolerance and high salt tolerance was screened from high temperature Daqu.