Objective To identify seed purity of south China type cucumber'' Chuanlv15'' quickly，simply and accurately，Method 240 SSR molecular markers evenly distributing in cucumber genome were screened for polymorphism and purity identification.Result Two SSR molecular markers （Cs100 and Cs109） showed diversity between parents and were selected to identify the seeds purity of ''Chuanlv15''. The identification result using SSR markers was consist with the results from morphology in the field，and the two SSR markers could separate the ''Chuanlv15'' seeds from other varieties of seeds.Conclusion In this study， we established a quick， easy and accurate technical method to identify the seed purity of ''Chuanlv15'' with two specific SSR molecular markers（Cs100 and Cs109）， which could supply the technical support for the seed commercialization and large extension of ''Chuanlv15''.Keywords：SSR molecular marker； cucumber； ''Chuanlv 15''； purity identification