The effects of different mass concentrations (0, 0.2, 0.5, 1.0, 10.0, 15.0, 20.0, 40.0 mg/L) of CeCl₃ on the in the proliferation, root growth, agronomic traits and physiological indexes of tissue culture seedlings of ‘Red Face’ strawberry were studied by tissue culture technique. The results showed that : (1) After applying 0.5 mg/L CeCl₃, the proliferation coefficient of strawberry tissue culture seedlings was the largest, the fresh weight was significantly higher than other treatments, and the root length was the longest. (2) After applying 10 mg/L CeCl₃, the rooting effect of tissue culture seedlings of strawberry was the best, the root surface area was the largest, the number of root tips was the largest, the plant was the highest, the leaf area and SPAD were the largest. (3) The activity of peroxidase （POD） and superoxide dismutase （SOD） showed a trend of '' first increase and then decrease '' with the increase of CeCl₃ concentration. When the concentration of CeCl₃ was 0.5 mg/L, the activity of POD was the strongest, and the activity of SOD was the strongest when the concentration of CeCl₃ was 20 mg/L. CeCl₃ had a certain promoting effect on the in vitro regeneration of strawberry tissue culture seedlings, but high concentration of CeCl₃ inhibited the growth of strawberry tissue culture seedlings.