[Objective]Accurately determine the concentration of Ralstonia solanacearum. [Method] Tryptone soy broth medium and physiological saline were used as diluents to dilute Ralstonia solanacearum RsA( tobacco source) and RsB (tobacco source).The concentration of bacterial suspension was measured by hemocytometer method,and the absorbance value of bacterial suspension at 600 nm was measured by spectrophotometer.The linear relationship between the concentration of Ralstonia solanacearum and the absorbance value was established.[Result]Linear regression equation demonstrated that there was no significant difference in slopes between two diluents.However,the absorbance values of RsA and RsB bacterial suspensions were significantly different,and the regression equations were y = 6.578 4x+0.158 28(r2 = 0.998 99) and y= 5.162 7x-0.125 82(r2=0.998 99) respectively.[Conclusion]The concentration standard curve of Ralstonia solanacearum established in this study can be used to determine the absorbance value and quickly calculate the concentration of Ralstonia solanacearum suspension from different sources without replacing the bacterial suspension medium with normal saline for absorbance determination.The method reported in the study was provided with convenience and practicality.