To understand the biological traits and drug resistance of pathogenic Escherichia coli from chicken, we conducted the study by collecting disease materials, bacterial isolation and culture, 16S rRNA gene identification, pathogenicity test, drug sensitivity test and other tests. The results show that the culture and microscopic traits of the 20 isolates are consistent with the traits of E. coli; the 16S rRNA gene sequence was 99.9%-100% homologous to E. coli by BLAST, and 20 isolates were identified as chicken-derived Escherichia coli; 20 strains of Escherichia coli have a pathogenicity of 40% to 100%; 10 strains of chicken-derived pathogenic Escherichia coli have multi-drug resistance to 15 antimicrobial agents, and the resistance rate is 33.3%-93.3% . The drug-resistant situation is serious and complicated