In view of the current situation of poor teaching effect of Statistics in application-oriented undergraduate universities, a questionnaire survey was carried out at Putian University, and the data of which is used for the empirical analysis. Preliminary statistical analysis shows that students have limited knowledge of the use of statistics, and are not working hard enough; meanwhile, Statistics teaching and assessment put too much emphasis on theory, but lack practical application. Factor analysis shows that multiple variables affecting teaching effect can be reduced to five factors: teachers’teaching level, students’learning input, professional contact degree, practical teaching and teaching materials. Regression analysis shows that these five factors have significant impact on teaching effect. Finally, some suggestions are put forward: improving teachers' teaching level, changing students' cognition of statistical use, and increasing practice proportion.