Abstract:The antioxidant activity of dichloromethane fraction and ethyl acetate fraction of Syzygium buxifolium leaf was compared by measuring of DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical scavenging capacity, reducing power, effects of metal ions on antioxidant activity and total phenolic content. The results showed that the EC50 value of DPPH radical scavenging capacity by dichloromethane fraction and ethyl acetate fraction from Syzygium buxifolium leaf was 0.63 mg/mL and 0.10 mg/mL, respectively. The reducing power EC50 value of dichloromethane fraction and ethyl acetate fraction was 0.87mg/mL and 0.09 mg/mL, respectively. In addition to Al3 + and Fe3 + , metal ions including K+, Na+, Mn2+, Mg2+, Cu2+, Zn2+, Ca2+ had inhibitory effect to DPPH radical scavenging by ethyl acetate fraction, while metal ions including K + ,Na + ,Mn2 + ,Mg2 + ,Al3 + ,Zn2 + ,Fe3 + had enhancement effect to DPPH radical scavenging by dichloromethane fraction except Cu2 + ,Ca2 + .Total phenolic content of dichloromethane fraction and ethyl acetate fraction was 9.23% and 59.17% , respectively. These results indicate that ethyl acetate fraction of Syzygium buxifolium leaf has stronger antioxidant activity and higher total phenolic content, but its antioxidant activity may be prone to antagonism of metal ions.