The influence of culture conditions on Mucor intracellular metabolites stability was investigated in this paper. A Mucor strain was cultured in different medium, by different inoculation volume, for different culture time, at different culture temperature and different shaking speed, and then the cultures were detected on HPLC after extracting by 50% methanol solution. Through analysis of HPLC fingerprints, the standardized training process was established: potato dextrose was chose to be the medium, and the medium was packed to 18 x 180 mm test tube (30mL/ tube). 2 mL of the spore suspension was inoculated in the medium after sterilization, and then the tube was placed in thermostatic shaker to culture for 4 d at 20 ℃ and 150 r/min