Using the biodiversity theory，this paper evaluated the biodiversity of Qionghai-wetland watershed through evaluation index system and value-determined standard. Results indicated that the biodiversity of Qionghai-wetland is preferable，species in the area is rich，endemic genus is superior，ecological structure is complex and many types of habitat.In addition，“habitat development strength”index score is relatively low，suggests that intrusion from human activities of Qionghai-wetland is serious，especially damage and intrusion from agricultural activities and tourist activities of Qionghai-wetland are bigger.