The Avian influenza maternal antibody and HI antibody of duck were detected to institute scientific immune program. Avian influenza oil emulsion vaccine of H5 and H9 were used in the test. The results showed that the maternal antibody level was highest in 1-day-age duck and cut down gradually. The maternal antibody of H5 in duck was decreased to 4.5log2 after 20-day-age and maternal antibody of H9 was decreased to 4.8log2 after 25-day-age. The duck could get high HI antibody level of H5 and H9 by first immunized in 8-day-age and several immunity. The immune programs used in the test could usefully prevent avian influenza in duck.