Several impotant factors influencing transformation frequency of rice mediated by Agmbacterium were researched with seven indica and japonica rice cultivars such as SH881,SH527,ZH9 and so on.For indica rice,CC medium is the best for callus initiation.For japonica rice,NB medium is the best for callus initiation.Lead ABA in the culture not to improve the initiation rate of the mature embryo of indica rice and japonica rice notably, But can obviously improve and injure quality in ABA for 3mg/L; The proper low-temperature pretreatment can improve the initiation rate; The transformation ability of Agrobacterium strain EHA105 is superior to LBA4404 and AGL1 to rice; While differention , it helps to improve the differentiation rate to adopt suitable hormone combination.