[Objective] To provide a theoretical basis for screening of Armillaria mellea that is highly compatible and co-living with Polyporus umbellatus. [Method] The mycelial growth of the combinations was studied in different medium, temperature and pH after co-cultivation on a plate for screening out the most suitable culture conditions. [Results] The mycelium of Polyporus umbellatus and Armillaria mellea grew well and fast in the improved PDA medium，25℃ , pH 6.5 and 24 hours dark. [Conclusion] This provided a theoretical basis for establishment of a co-culture system of Polyporus umbellatus and Armillaria mellea, and for screening of key factors for Polyporus umbellatus's highly compatible Armillaria mellea.