Objective It is aimed to optimize the culture conditions for amylase and protease production by Bacillus thuringiensis isolated from high-temperature Daqu.Method A combination of single-factor test and response surface methodology （RSM） was adopted. The effects of such factors as carbon source， nitrogen source， their mass concentrations， and liquid loading volume on the enzyme-producing activity of B. thuringiensis were systematically analyzed. Specifically， different carbon sources， nitrogen sources， and their concentrations were tested. Also， various liquid loading volumes were investigated. On the basis of the single-factor test results， RSM was further used to determine the optimal culture conditions for enzyme production.Result When the medium composition was 21 g/L of yeast powder， 32 g/L of soluble starch， 95 mL/250 mL of liquid loading volume， and 5%（volume fraction） of inoculum size， at 37 ℃， pH 7.0， and 150 r/min， the amylase activity reached 349.32 μmol/（min·mL）， which was 5.4 times that before optimization； when the medium composition was 12 g/L of yeast powder， 22 g/L of soluble starch， 95 mL/250 mL of liquid loading volume， and 5%（volume fraction） of inoculum size， at 37 ℃， pH 7.0， and 150 r/min， the protease activity was 8.70 µmol·min^-1·mL^-1， which was 14.5 times that before optimization.Conclusion Through the comprehensive application of single-factor experiments and RSM， the amylase and protease production capabilities of B. thuringiensis were successfully increased by 5.4 times and 14.5 times respectively.