一株根际多功能解磷菌的分离,筛选及鉴定
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1.西昌学院,资源与环境学院,四川 西昌 615013;2.西昌学院,动物科学学院,四川 西昌 615013;3.四川省烟草公司凉山州公司 盐源分公司,四川 盐源 615703

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凉山州科技计划项目(21ZDF0181);西昌学院“两高”人才科研支持计划项目(LGZ202001);国家大学生创新计划项目(202210628023);四川省大学生创新训练项目(S202310628086)。


Isolation, Screening, and Identification of a Multifunctional Phosphate-Solubilizing Bacterium from Rhizosphere Soil
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1.aCollege of Resources and Environment; 1b.College of Animal Science, Xichang University, Xichang 615013,Sichuan, China;2.Sichuan Tobacco Industrial Co., Ltd., Liangshan Prefecture Company,Yanyuan Branch, Yanyuan 615703, Sichuan, China

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    摘要:

    目的 旨在从根际土壤中筛选高效解磷菌,以解决土壤中难溶性无机磷和有机磷的富集问题,同时筛选具有潜在植物促生特性的菌株。方法 采用蒙金娜培养基对根际土壤样本进行筛选分离,结合形态学特征、生理生化特性及16S rDNA基因鉴定对菌株L17进行系统鉴定;通过钼锑抗比显色法测定解磷能力,火焰原子吸收法测定解钾能力,碱性过硫酸钾消解法测定固氮能力,Salkowski显色法测定吲哚乙酸(IAA)分泌能力。结果 菌株L17属于芽孢杆菌属(Bacillus sp.),其解磷能力为(78.436±0.224) μg/mL,解钾能力为(58.900±2.390) μg/mL,固氮能力为(106.610±4.580) μg/mL,吲哚乙酸分泌量为(71.711±1.812) μg/mL。结论 菌株L17具有显著的解磷、解钾、固氮及分泌吲哚乙酸能力,展现出较强的植物促生潜力,可为土壤修复复合菌剂的开发及环境污染生物降解菌剂的研究提供支持。

    Abstract:

    Objective This study aimed at screening high-efficiency phosphate-solubilizing bacteria from rhizosphere soil to address the accumulation of insoluble inorganic and organic phosphorus in soil, while identifying strains with potential plant growth-promoting properties.Method The strain L17 was isolated and screened using Pikovskayas Medium, followed by systematic identification based on morphological characteristics, physiological and biochemical properties, and 16S rDNA gene analysis. The phosphate-solubilizing ability was determined by the molybdenum-antimony anti-colorimetric method, potassium-releasing ability by flame atomic absorption spectrometry, nitrogen-fixing ability by alkaline persulfate digestion, and indole-3-acetic acid (IAA) secretion by the Salkowski colorimetric method.Result The experimental results showed that strain L17 belongs to the genus Bacillus (Bacillus sp.). Its phosphate-solubilizing ability was (78.436±0.224) μg/mL, potassium-releasing ability was (58.900±2.390) μg/mL, nitrogen-fixing ability was (106.610±4.580) μg/mL, and IAA secretion reached (71.711±1.812) μg/mL.Conclusion Strain L17 exhibits significant phosphate-solubilizing, potassium-releasing, nitrogen-fixing, and IAA-secreting abilities, demonstrating strong potential for promoting plant growth. It provides important support for the development of soil remediation microbial agents and research on biodegradation agents for environmental pollution.

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周星朴,沈依华,张文丽,黄诗维,王洁,陈梦越,孙劲.一株根际多功能解磷菌的分离,筛选及鉴定[J].西昌学院学报(自然科学版),2025,39(1):25-32.

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  • 收稿日期:2025-01-21
  • 最后修改日期:2025-02-06
  • 录用日期:2025-02-09
  • 在线发布日期: 2025-05-16