2种药剂对水稻愈伤组织分化的影响及分化苗抗除草剂鉴定
作者:
作者单位:

1.安徽科技学院农学院,安徽 凤阳233100;2.安徽省饲草生物育种国际合作研究中心,安徽 凤阳233100

作者简介:

通讯作者:

基金项目:

安徽省重点研究与开发计划(202204c06020069)。


Effects of Two Chemicals on the Differentiation of Rice Callus and Identification of Herbicide Resistance of the Differentiated Seedlings
Author:
Affiliation:

1.College of Agriculture, Anhui Science and Technology University,Fengyang233100, Anhui, China;2.Anhui International Cooperative Research Center for Forage Bio-breeding, Fengyang233100, Anhui, China

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
    摘要:

    目的 通过对水稻愈伤组织进行化学诱变与除草剂筛选,鉴定具有抗除草剂的水稻新种质资源和乙酰乳酸合成酶(ALS)基因新位点。方法 研究了诱变剂叠氮化钠(NaN3)和除草剂甲氧咪草烟对水稻愈伤组织诱导分化的影响,并对分化苗进行了除草剂抗性鉴定和分子鉴定。结果 NaN3处理后可提高愈伤组织在甲氧咪草烟处理下的分化率,且在0.005 g/L甲氧咪草烟溶液、2 mmol/L NaN3溶液处理下,愈伤组织分化率最高,其分化率为33.30%。利用不同质量浓度的甲氧咪草烟溶液对分化苗进行筛选,在田间推荐剂量1倍(3.16 g/L)和2倍(6.32 g/L)质量浓度甲氧咪草烟溶液喷施下均有分化苗存活,且在2倍处理下存活率达到20%。对抗性苗进行重测序表明,ALS基因第199位的碱基C突变为A,导致第67位氨基酸由脯氨酸变为苏氨酸。结论 利用NaN3及甲氧咪草烟筛选可以获得抗甲氧咪草烟水稻新种质,并且获得的Pro67Thr的突变为水稻抗甲氧咪草烟育种新位点,这为抗除草剂诱变育种研究提供了种质资源和基因资源。

    Abstract:

    Objective Rice callus were screened by chemical mutagenesis and herbicide, and new rice germplasms resources with herbicide resistance and new loci of acetolactate synthase (ALS) gene were identified.Method In this study, the effects of sodium azide (NaN3) and imazamox on the differentiation of rice callus were analyzed. And the herbicide resistance identification and molecular identification were performed the differentiated seedlings.Result The NaN3 treatment could increase the differentiation rate when the medium was added NaN3. The highest differentiation rate was 33.30% on the treatment of 0.005 g/L imazamox solution and 2 mmol/L NaN3 solution. The differentiated seedlings were screened using different concentrations of imazamox. The differentiated seedlings were sprayed with 1 times (3.16 g/L) and 2 times (6.32 g/L) concentration of imazamox, and their survival rate reached 20% under the treatment of twice the recommended concentration. It proved that these survived seedlings had resistance to imazamox. The resistant seedlings were sequenced by second generation sequencing. It showed that the base C was mutated to A at position 199 of the ALS gene, resulting in a change from proline to threonine at the position.Conclusion New rice germplasm resistant to imazamox were obtained. The mutation of Pro67Thr is a new resistant locus to imazamox. Our research provides germplasm resources and genetic resources for the study of rice herbicide-resistant mutagenesis breeding.

    参考文献
    相似文献
    引证文献
引用本文

程正宵,李杰勤,钱晶晶,唐靖雯,张从宇.2种药剂对水稻愈伤组织分化的影响及分化苗抗除草剂鉴定[J].西昌学院学报(自然科学版),2024,38(4):13-20.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
历史
  • 收稿日期:2024-11-09
  • 最后修改日期:2024-11-22
  • 录用日期:2024-12-05
  • 在线发布日期: 2025-01-24