Objective Obtain a lipase-producing strain with strong enzyme production ability and environmental safety.Method The rhodamine B plate method was used to screen high-lipase-producing strains from oil-contaminated soil and water. The safety of the strains was determined by blood plate inoculation and antibiotic sensitivity test. The fermentation enzyme production conditions of the strains were optimized by nitrogen source， carbon source and inducer single factor experiment combined with response surface methodology. The enzymatic characteristics of the lipase produced by the strains were analyzed.Result A strain JC-7 with high lipase production ability was isolated and screened from oil-contaminated soil. It was identified as Serratia sp. The strain was γ-hemolytic on blood agar plate and sensitive to most antibiotics. After optimization by response surface methodology， the enzyme activity reached up to 38.6 μmol/min. The lipase produced by strain JC-7 is a mesophilic alkaline lipase. Ca²⁺ and Mg²⁺ can improve the enzyme activity. The enzyme can maintain high activity in glycerol， dimethyl sulfoxide and methanol. Sodium dodecyl sulfate（SDS）， hexadecyl trimethyl ammonium bromide（CTAB） and TritonX-100 all activated the enzyme， dithiothreitol（DTT） had almost no effect on the enzyme activity， and ethylene diamine tetraacetic acid（EDTA） inhibited the enzyme activity.Conclusion The isolated Serratia JC-7 has a strong ability to produce lipase， is safe and friendly to the environment， and the enzyme production capacity is significantly improved after optimizing the culture conditions. The enzyme can adapt to a wider range of action conditions， which can provide support for the development of lipase preparations and the research on environmental pollution biodegradation agents.