The acute toxicity of povidone iodine to Gymnocypri sprzewalskii （♀） × Gymnocypris eckloni （♂） hybrid larvae （7-8 cm， 15.34 g±0.23 g） was investigated， and the effects of different concentrations of povidone iodine on the oxygen consumption rate of Gymnocypri sprzewalskii （♀） × Gymnocypris eckloni （♂） hybrid larvae were also determined. The results showed that ：（1） At the water temperature of （19±1）℃， the median lethal concentration （LC₅₀） of povidone iodine were 13.37， 3.15， 2.75和2.51 μmol/L for hybrid larvae at 24， 48， 72 and 96 h， respectively， and the safe concentration was 0.84 μmol/L； （2）The oxygen consumption rates of hybrid larvae were 0.340 mg/（g·h） and 0.293 mg/（g·h） at the safe concentration （0.84 μmol/L） and the production concentration （0.22 μmol/L）， respectively， and the asphyxiation points were 0.417 mg/L and 0.383 mg/L， which were all significantly higher than the oxygen consumption rate 0.243 mg/（g·h） and asphyxiation point 0.363 mg/L of hybrid larvae without povidone iodine（P<0.05）.