Objective The selenium content of 87 Tartary buckwheat seeds from different sources was determined by wet digestion and fluorescence spectrophotometry in order to identify the resources of Tartary Buckwheat with high selenium content and further select and cultivate the seed rich in selenium.Methods The selenium content of 87 Tartary buckwheat seed resources was determined by wet digestion-fluorescence spectrophotometry with the recovery rate and digestion time as the evaluation indexes.Results At four digestion temperatures， the recovery of selenium was 87.2%-102.4%. The higher the digestion temperature is， the shorter the digestion time； whereas the higher the temperature is， the easier the sample is to explode and evaporate locally， which reduces the recovery rate. Considering all factors， the digestion temperature is selected at 240℃. The standard deviation of selenium content in 10 Tartary buckwheat seed samples was 0.45 by wet digestion and fluorescence spectrophotometry. The selenium content of 87 Tartary buckwheat seeds varied from 0.0109mg/kg to 0.1790 mg/kg， with an average of 0.0560mg/kg.Conclusion 42 varieties of Tartary buckwheat with higher content （> 0.0500 mg/kg） were selected， which provided a theoretical basis for the study of selenium-rich Tartary buckwheat products.