Animal Breeding Course is an important basic course for Animal Science major. Animal Breeding Course has the characteristics of complex knowledge structure and strong practicality. The traditional “teacher-centered” teaching mode can’t meet the demand of qualified and skilled talents by the development of animal husbandry，and the teaching quality and student training quality need to be improved by relying on the teaching reform. PBL （Problem-based learning） teaching mode was used in the teaching of Animal Breeding Course in Gansu Agricultural University. A questionnaire survey was conducted to investigate students'' satisfaction with PBL teaching mode. Statistical analysis was performed for students'' experimental scores，usual scores，final scores and comprehensive scores. Thus，we objectively evaluate the effect of PBL teaching mode in the teaching reform of Animal Breeding Course. The results showed that the average scores of students'' experimental scores，usual scores，final scores and comprehensive scores in PBL teaching mode were significantly higher than those in traditional teaching mode. Most of the students scored above 80 points in PBL teaching mode. The questionnaire survey found that 96.67% of the students supported PBL teaching mode. PBL teaching mode can significantly improve students'' interest in learning and promote their command of professional knowledge and application ability in Animal Breeding Course.