Seed purity is a key index to measure the quality of seeds， and the identification of seed purity is also a crucial step in the commercial breeding process of cucumber hybrid. ‘Chuancui13’ is a new cucumber cultivar bred by cucumber research group of Horticultural Research Institute， Sichuan Academy of Agricultural Sciences. To identify the purity of hybrid seeds quickly， simply and accurately， the simple sequence repeat （SSR） markers showing complementary bands pattern between the parents were screened out in 240 SSR molecular markers. The accuracy of SSR markers was verified by field morphological identification. The results show that ：（1）two pairs of SSR markers （Cs007 and Cs087） show polymorphism differences between two parents； （2）the accuracy verification results are consistent with the field morphological identification results. The results show that the two pairs of SSR markers （Cs007 and Cs087） are the specific markers to identify seed purity identification of ‘Chuancui13’. A technical method of the purity identification of ‘Chuancui13’ hybrid seeds is constructed using specific SSR markers Cs007 and Cs087 as the core primers. It has greatly shortened the time of hybrid purity identification， improved identification efficiency， and provided technical support for the commercialization breeding in cucumber.