Objective The aim was to clarify the control effect of seven fungicides and antagonistic bacteria SJ1606 on Valsa mali, screen out efficient mixed fungicides, and provide reference for the reduction and synergistic use of traditional chemical fungicides.Method The inhibitory effects of seven fungicides and antagonistic bacteria SJ1606 fermentation metabolites and new mixtures on Valsa mali were determined by mycelial growth rate method.Result The results showed that V.mali was highly sensitive to prochloraz, tebuconazole, propiconazole, carbendazim and difenoconazole. The EC₅₀ values of the above five chemical fungicides were 0.12, 0.33, 0.57, 0.64， 1.14 μg / mL, respectively. The EC₅₀ of moderate sensitivity to pyraclostrobin was 6.92 μg/mL. It was insensitive to thiram, and the EC₅₀ was 149.99 μg/mL. The EC₅₀ of SJ1606 fermentation supernatant and lipopeptide crude extract were 40.36， 14.75 μL/mL, respectively. The crude extract of lipopeptide showed a strong synergistic effect on some chemical agents by volume mixing method, and the synergistic effect was the most obvious when mixed with carbendazim at 7∶3, with a toxicity ratio of 1.72. The highest toxicity ratio with pyraclostrobin at 6∶4 was 1.58, and the highest toxicity ratio with prochloraz at 6∶4 was 1.50. The highest toxicity ratio was 1.48 when mixed with propiconazole at 5∶5.Conclusion The synergistic ratio of lipopeptide crude extract to chemical fungicides was successfully screened out, which provided technical support for the development and utilization of biocontrol bacteria SJ1606, the reduction of fungicides and the creation of new mixtures.