The solid and liquid culture conditions of Streptococcus salivarius were optimized by single factor test and referring to existing literature. The optimum conditions for solid culture of Streptococcus salivarius were obtained as follows: MRS medium, pH7.2, temperature 37 ℃ in incubator containing 5% CO2. The optimum conditions for liquid culture of Streptococcus salivarius were obtained as follows: according to the ratio 7 g of lactose, 3.5 g of sucrose,3.5 g of glucose,14 g of tryptone,7 g of soybean peptone,7 g of yeast extract,0.02 mol/L of potassium dihydrogen phosphate,200 mg/L of magnesium sulfate,100 mg/L of manganese sulfate,60 mg/L of calcium chloride, 10 mg/L of VitB1, distilled water 1L to prepare the liquid culture medium, pH 7.2, temperature 37℃ in incubator containing 5% CO2. After enlarged culture, the final lyophilized bacterial powder was 10.9 g, and the viable bacterial count was 4.9×1011cfu/g. It provides effective information for industrial production.