Using methods combining questionnaire,interview and literature study,this paper expounds the causes for the lack of interest in PE lesson among female students in Anhui’s higher vocational colleges from the aspects of the female students’cognition to the body-building function of PE,the teaching content of PE lesson,teaching hardware,score evaluation standard,PE teachers’basic quality and so on and then advances some operable countermeasures against the phenomenon such as fostering the female students’thought of lifelong sports,providing more physical specialized courses reasonably,improving the sports environment,optimizing the score evaluation system of PE course and enhancing PE teachers’comprehensive quality so as to provide a reference to higher vocational colleges to strengthen their PE teaching on female students.