The sampling survey matching with the Overall Subjective Well-Being List and Work Satisfaction List is applied in this paper. The survey aims to carry out a probe into current situation of the subjective well-being of Yi and Han teachers in Yi Region of Liangshan and provide some references for keeping abreast of the psychological situation and life quality of Yi and Han teachers in this region. The overall subjective well-being of the Yi and Han teachers in Yi region of Liangshan is in a lower level. But in this region, the subjective well-being of Yi teachers is higher than that of the Han teachers and the teaching work satisfaction is in the up middle level and related with the subjective well-being. There are perceptible differences between the Yi and Han teachers in Yi region of Liangshan with respect to the subjective well-being. Based on the study, it is found that key factors that produce these differences come from the traditional cultural adhesion, nationality belongingness and sense of achievement, etc.