[Purpose] Understand the subjective well-being and mental health of college students with left-behind experience. And by exploring the relationship between their subjective well-being and mental health, it aims to provide support for the mental health education of college students who have left-behind experience.[Method] Subjective well-being scale, Symptom Checklist 90 (SCL-90) and self-prepared left-behind questionnaire were used to investigate students. [Result] Students who do not have left-behind experience have higher levels of subjective well-being and mental health than students who have left-behind experience. And in the survey of the college students who have left-behind experience, their total score of subjective well-being has a significant negative correlation with the total score of mental health and its various dimensions. [Conclusion] There is a significant positive correlation between the level of subjective well-being and the level of mental health of college students with left-behind experience. Therefore, we must pay attention to the improvement of subjective well-being of college students with left-behind experience with a view to improve the mental health of college students.