文章摘要
任 丽1 ,徐 睿1,2* ,唐 强1 ,何欣怡1.鸡源致病性大肠杆菌的分离鉴定及药敏试验[J].西昌学院学报(自然科学版),2019,33(1):22-27.
鸡源致病性大肠杆菌的分离鉴定及药敏试验
A Study on Isolation Identification and Drug Sensitivity Test of Chicken-derivedPathogenic Escherichia Coli
  
DOI:
中文关键词: 鸡源大肠杆菌  分离鉴定  致病性  药敏试验
英文关键词: chicken-derived Escherichia coli  isolation and identification  pathogenicity  drug sensitivity test
基金项目:四川省凉山州农业科技创新项目(15NYCX0040);四川省科技厅支撑计划项目(13ZC1796);四川省教育厅攀西动 物疫病防控与检测高校重点实验室(纵341B)
作者单位
任 丽1 ,徐 睿1,2* ,唐 强1 ,何欣怡1 1. 西昌学院动物科学学院四川 西昌 6150013 2. 攀西动物疫病检测与防控高校重点实验室四川 西昌 615013 
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中文摘要:
      为了解鸡源致病性大肠杆菌的生物学特性和耐药性,试验通过采集病料、细菌分离培养、16S rRNA基因鉴定、致病性试 验、药敏试验等方法。结果表明:分离出的 20 株细菌的培养特征、镜检特征均符合大肠杆菌特征;16S rRNA 基因序列经 BLAST比对与大肠杆菌同源性达99.9%~100%,确定20株分离菌为鸡源大肠杆菌;20株大肠杆菌的致病力为40%~100%;10 株鸡源致病性大肠杆菌对15种抗菌药物均呈现多重耐药,耐药率为33.3%~93.3%,耐药情况较严重和复杂。
英文摘要:
      To understand the biological traits and drug resistance of pathogenic Escherichia coli from chicken, we conducted the study by collecting disease materials, bacterial isolation and culture, 16S rRNA gene identification, pathogenicity test, drug sensitivity test and other tests. The results show that the culture and microscopic traits of the 20 isolates are consistent with the traits of E. coli; the 16S rRNA gene sequence was 99.9%-100% homologous to E. coli by BLAST, and 20 isolates were identified as chicken-derived Escherichia coli; 20 strains of Escherichia coli have a pathogenicity of 40% to 100%; 10 strains of chicken-derived pathogenic Escherichia coli have multi-drug resistance to 15 antimicrobial agents, and the resistance rate is 33.3%-93.3% . The drug-resistant situation is serious and complicated
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