| 徐洲,达小梅.PMP柱前衍生HPLC法测定松乳菇多糖的单糖组成[J].西昌学院学报（自然科学版）,2023,37(3):1-6. |
| PMP柱前衍生HPLC法测定松乳菇多糖的单糖组成 |
| Determination of Monosaccharide Composition of Lactarius Deliciosus Polysaccharides by PMP Precolumn Derivatization HPLC |
| 投稿时间：2023-07-17 修订日期：2023-08-29 |
| DOI：10.16104/j.issn.1673-1891.2023.03.001 |
| 中文关键词: PMP HPLC 松乳菇多糖 单糖 |
| 英文关键词: PMP HPLC lactarius deliciosus polysaccharides monosaccharides |
| 基金项目:攀西特色作物研究与利用四川省重点实验室发展项目基金（XNFZ2107\SZKF2212）。 |
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| 中文摘要: |
| 建立了一种1-苯基-3-甲基-5-吡唑酮（PMP）柱前衍生高效液相色谱（high performance liquid chromatography，HPLC）法测定松乳菇多糖中单糖组成。通过单因素实验优化PMP衍生单糖的条件为反应时间40 min，反应温度70 °C和PMP浓度0.8 mol/L。以Agilent Zorbax SB-C18色谱柱为分析柱，磷酸缓冲液（0.05 mol/L，pH=6.9）-乙腈为流动相，检测波长为250 nm，进样量10 µL，柱温20 °C检测单糖组成。在此色谱条件下，各单糖线性关系良好（R2≥0.999 6），精密度高（RSD≤2.23%），重复性强（RSD≤2.11%），加标回收率为97.16%~101.68%。该方法适用于松乳菇多糖中单糖组成的测定。 |
| 英文摘要: |
| A high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) has been established for determination monosaccharides composition of polysaccharides purified from Lactarius deliciosus. The optimisation of the pre-columnderivatization process were: reaction time of 40 min, reaction temperature of 70°C and PMP concentration of 0.8 M. HPLC conditions were as follows: Agilent Zorbax SB-C18 column; using phosphate buffer (0.05 M，pH = 6.9)-acetonitrile as mobile phase; detection wavelength 250 nm; injection volume 10 μL; column temperature 20°C. The determination results had good linear correlations with R2 ≥ 0.9996), good accuracy (RSD ≤ 2.23%) and repeatability (RSD ≤ 2.11%), and the recovery rate was in the range of 97.16-101.68%. This method could precisely determinate monosaccharides composition of polysaccharides purified from L. deliciosus. |
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