贡嘎山不同海拔土壤中抗生素抗性基因的多样性特征
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四川省科技厅重点项目(2020YJ0346)?国家自然科学基金(41606142)?西华师范大学英才科研基金和留学博士后 基金(463140、416793)?


Diversity of Antibiotic Resistance Genes in Soils along
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    抗生素抗性基因作为一种新型环境污染物ꎬ对生态环境安全和人类健康有严重的威胁ꎮ 目前对抗生素抗性基因的研 究大多是对城市土壤、污泥、农田土壤等受人为干扰较多的环境开展ꎮ 然而ꎬ人为干扰少的土壤中抗性基因的背景值研究仍 较为缺乏ꎮ 针对贡嘎山不同海拔高度的土壤ꎬ采用高通量实时荧光定量 PCR 的方法ꎬ研究了其抗生素抗性基因和可移动遗传 元件的多样性及丰度特征ꎬ并探究其与环境因子的相关性ꎮ 结果发现:(1)贡嘎山海拔在 2 948 ~ 3 651 m 的 7 个土壤样品ꎬ共 检测到 132 种不同的抗性基因和 10 种可移动遗传元件ꎮ 每个样品中的抗性基因种数为 46~ 89 个ꎬ并随着海拔的升高呈增加 趋势ꎮ 抗性基因的相对拷贝数为 0.019~ 0.043 copies/ cellꎬ且不随海拔高度呈规律性变化ꎮ 7 个土壤中检测到 intI-1(clinic)和 tnpA-02 等可移动遗传元件ꎬ表明抗性基因的水平转移可能为这些土壤中抗性基因迁移的途径之一ꎮ (2)7 种土壤的抗性基 因种类组成差异有统计学意义(P<0.05)ꎬ冗余分析表明 NO3 - -N、NH4 + -N、含水量、海拔和有机质等土壤理化性质的差异可能 导致了不同土壤中抗性基因多样性的差异(解释率 97.1%)ꎮ 综上ꎬ受人为干扰较少的高原土壤中存在着多样性丰富的抗性 基因ꎬ该研究为抗生素抗性基因的污染研究提供了基础参考数据ꎮ

    Abstract:

    Antibiotic resistance genes (ARGs) are emerging pollutants which pose a serious threat to ecological environ ̄ ment and human health. Recentlyꎬ most of the ARGs studies are focus on the samples from urban soilsꎬ sludgeꎬ and agri ̄ cultural soils which are intensively influenced by humanity. Howeverꎬ the distribution of ARGs in the soils subjected to lit ̄ tle disturbance of human activities is largely unexplored. The high -throughput real -time fluorescent quantitative PCR method was used to analyze the diversity and abundance of ARGs and mobile genetic elements (MEGs) in the 7 soil sam ̄ ples from different altitude of the Gongga Mountain. The correlation between ARGs diversity and environmental factors was also determined. A total of 132 ARGs and 10 mobile genetic elements (MGEs) were detected in soil samples at an altitude of 2 948~ 3 651 meters in the Gongga Mountain. There were 46~ 89 ARGs subtypes per samples. The relative copy number in the 7 soil samples ranged from0.019 to 0.043 copies/ bacterial cellꎬ and did not change with the elevations. MEGsꎬ in ̄ cluding intI-1(clinic) and tnpA-02ꎬ were detected in all the 7 soil samplesꎬ suggesting that the horizontal transfer of ARGs contributed to the ARGs spread in the soils. The composition of the ARGs subtypes significantly differed among the 7 soil samples. Redundancy analysis showed that NO3 - -Nꎬ NH4 + -Nꎬ water contentꎬ altitude and TOC explained 97.1% of the differences in the distribution of ARGs in different altitudes of the Gongga Mountain. To sum upꎬ diverse and abundant ARGs exit in the natural and less disturbed soils. The study provides important data and reference to the ARGs pollution research.

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刘 燕ꎬ路 璐.贡嘎山不同海拔土壤中抗生素抗性基因的多样性特征[J].西昌学院学报(自然科学版),2021,35(2):1-7.

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  • 在线发布日期: 2021-07-27