一株纤维素降解菌的分离和鉴定
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四川省大学生创业创新项目(S201910628019);西昌学院两高人才计划项目(LGZ201916、LGZ201926);凉山州科技局资助项目(50161006)。


Isolation and Identification of a Cellulolytic Bacterium
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    摘要:

    为得到降解纤维素能力较强的细菌,以腐殖土壤为菌株来源筛选、分离纤维素降解细菌。对所取土样稀释一定比例进行涂布平板,以羧甲基纤维素钠(CMC-Na)为唯一碳源培养,用刚果红染色的方法筛选有透明圈的菌株,得到一株编号为YBX-52的纤维素降解细菌。在产酶培养基中以30 ℃培养48 h,用DNS法测得滤纸酶活力(FPA)为221.75 μmol/min、羧甲基纤维素酶活力(CMC)为274.56 μmol/min。以16 SrDNA通过序列对比,构建YBX-52系统发育树,结合其生理生化特征将其鉴定为苏云金芽孢杆菌(Bacillus thuringiensis)。

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    In order to obtain cellulose-degrading bacteria, humus soil isused as the strain source to screen and isolate cellulose-degrading bacteria. A cellulose degrading bacterium numbered YBX-52 is obtained from culturing CMC-Na as the sole carbon sourceon a plate coated with soil sample diluted in a certain proportion and screening the strain with transparent circle by Congo red staining. The filter paper enzyme activity (FPA) measured by DNS method is 221.75 mol/min and the carboxymethylcellulose enzyme activity (CMC) is 274.56 mol/min when cultured at 30 ℃ for 48 h in enzyme-producing medium. The YBX-52 phylogenetic tree is constructed by sequence comparison with 16SrDNA, and identified as Bacillus thuringiensis according to its physiological and biochemical characteristics.

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周思才,石家城,高瑞佳,艾 爽,罗 鹏,王中丽,孙 劲.一株纤维素降解菌的分离和鉴定[J].西昌学院学报(自然科学版),2020,34(4):5-7.

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  • 在线发布日期: 2021-01-21