文章摘要
赵 梅a,b,杨俊宝b*.视网膜母细胞瘤结合蛋白4基因酵母双杂交诱饵载体的构建[J].西昌学院学报(自然科学版),2019,33(2):17-19.
视网膜母细胞瘤结合蛋白4基因酵母双杂交诱饵载体的构建
Construction ofYeast Two-Hybrid Bait Vector pGBKT7-RBBP4 ZHAO Meia,b,YANG Junbaob*
  
DOI:
中文关键词: 酵母双杂交  诱饵载体  RBBP4基因
英文关键词: yeast two hybrid  bait vector  RBBP4 gene
基金项目:四川省教育厅重点项目(18ZA0198);四川省卫计委重点项目(18ZD038);川北医学院博士科研启动基金 (CBY17-QD10);南充市科知局市校合作项目(18SXHZ0232)。
作者单位
赵 梅a,b,杨俊宝b* 川北医学院a.人事处 b.医学遗传学与细胞生物学教研室/医学遗传中心四川 南充 637100 
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中文摘要:
      视网膜母细胞瘤结合蛋白4(RBBP4)是WD-40蛋白家族的成员之一,其在组蛋白乙酰化、细胞周期进展及肿瘤干细胞 的分化等方面具有重要功能,研究与RBBP4互作的新蛋白质和认识RBBP4在疾病包括肿瘤的发生发展过程中的分子机制有 重要意义。通过 RT-PCR 扩增获得了 RBBP4 基因的 CDS 序列,经双酶切后与 pGBKT7 酵母诱饵载体连接构建了 pGBKT7-RBBP4载体,再通过双酶切鉴定和测序验证,结果表明此酵母双杂交诱饵载体被成功构建,为后续利用酵母双杂交 技术筛选与RBBP4蛋白相互作用的新蛋白提供了参考。
英文摘要:
      Being a member of WD-40 protein family, retinoblastoma binding protein 4 (RBBP4) plays an important role in histone acetylation, cell cycle progression and differentiation of cancer stem cells. It is important to study new proteins interacting with RBBP4 and to understand its molecular mechanism in the pathogenesis of diseases, including tumors. In this study, the RBBP4 gene CDS sequence was successfully amplified by RT-PCR, and pGBKT7-RBBP4 was constructed by binding with pGBKT7 yeast bait vector. The results of double enzyme digestion and sequencing confirmed that the bait vector of yeast two-hybrid was successfully constructed, which provided a reference for screening new protein interacting with RBBP4 protein by yeast two-hybrid technology.
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