[Objective]To guarantee the root quality of Aconite and to create a quality testing procedure and grading criteria for the roots of Aconite in Sichuan. [Method] Through studies of the six indicators of aconite root authenticity, purity, water content, germination rate, disease rate and weight of 100 units to establish the quality testing procedure for the roots of Aconite; through tests of 30 aconite roots and adoption of the K-means clustering method to establish the grading criteria for aconite roots. [Results] Water content and germination rate are major grading criteria for aconite roots; purity, disease rate and weight of 100 units are secondary grading criteria. The aconite roots are graded in three categories: first-grade root has germination rate ≥70% , water content of 65%-70% , purity ≥ 90% , hundred units weight of 1 100-1 500 g, and diseased plant rate ≤ 4.5%; second-grade root has germination rate ≥ 70%, water content of 65%-70%, purity ≥90%, hundred units weight of 900-1 099 g, and diseased plant rate ≤ 4.5%; third-grade seedling has germination rate ≥60%, water content of 71%-75%, purity ≥85%, hundred units weight of 600-899 g, and diseased plant rate ≤4.5%.[Conclusion] In this study, a lot of experimental data were used to establish the rules for the root examination of Aconitum carmichaeli Debx. and to establish classification standards.