In order to further study the role of CDIO engineering education concept in the field of physical education, to investigate the effect of CDIO teaching model under the concept of physical and mental health of female college students, this paper adopts the literature method to build and design the "cooperation and interaction" teaching based on the CDIO concept, and then apply it into 16 weeks of physical education. The teaching experiment tests were carried out on 80 female students randomly selected from Grade 16, combining with the experimental method, questionnaire survey method, mathematical statistics and other methods. Through the comparison and analysis of the changes of students' physical and psychological indicators index, the author would like to explore the influence of the"cooperation and interaction" teaching on the physical and mental health of female college students.