In view of strong practical characteristics of the MCU course and the demand for training of innovative talents, the author designs an innovative experiment system based on ATmega16 MCU and its application and teaching practice. The curriculum is based on practice, supplemented by theory, with students as the main part, teachers as the auxiliary, the principle of practical operation and virtual simulation as the supplement. Through the design and development of the ATmega16 MCU experiment system, the students have mastered the design of the system schematic diagram, the PCB board design, the welding debugging and the software program design. It aims to guide students to discover problems and solve problems through practical links, to highlight the practicality, engineering and application of singlechip, and to cultivate students' practical application and innovation ability