The effects of substrate, growth regulator type, and growth regulator treatment type on rooting of hardwood cuttings of Mirabolano 29C were studied through the orthogonal experimental and complete randomized trial method. The results were as follows: soaking the base of the plant cuttings in 100 mg/L IBA solution for 6 hours then inserted to the rooting medium perlite gave rise to rooting rate of 84.62%, the average root number was 10.87 and the average root length was 3.84 cm; dipping the base of plant cuttings in 1 000 mg/L IBA solution for 30 s then inserted to the rooting medium perlite gave rise to rooting rate of 89.1%.