In this paper, the effect of“3414”fertilizer efficiency experiment on Chlorophyll content of flue-cured tobacco NC102 was studied. The test results showed that the different fertilizer formulas treatments had remarkable influence on the Chlorophyll content of tobacco. The amount of nitrogen was closely related with Chlorophyll content of tobacco. When the fertilizing amount of phosphorus and potassium were the recommended levels, the Chlorophyll content of tobacco increased with the nitrogen of topdressing increased.The Chlorophyll Content of tobacco was the highest when the fertilizing amount of nitrogen was at level 3(135 Kg/hm2); When the fertilizing amount of phosphorus and nitrogen were the recommended levels, increasing potassium would lead to an increase in Chlorophyll content of tobacco.But continuing to increase potassium such as at level 3(337.5 Kg/hm2), the Chlorophyll content of tobacco would decline sharply; The study showed that the effect of the fertilizing amount of phosphorus on Chlorophyll content of tobacco was few, while a large amount of potassium would decline it sharply