Pomegranate wilt is a fungal disease caused by Ceratocystis fimbriata，which was a new diseasedevastated in Sichuan Panzhihua-Xichang area. The biological characteristics of C. fimbriata were studied. The results showed that the mycelia could grow well in sweet potato dextrose agar（SPDA），sweet potato and potato dextrose agar，potato dextrose agar（PDA），corn meal agar and pomegranate leaf juice culture media，but never in the Czapek culture media or 2％ water agar culture media. C. fimbriata could utilize carbon sources，and dextrose was the optimum. The mycelial growth was impacted by different temperature and pH. The temperature for mycelial growth ranged from 10℃ to 35℃，the optimum temperature was 26℃ and the lethal temperature of the mycelium was 50℃ for 10 minutes. The mycelia could grow from pH3 to pH6，and pH5 was the optimum. The dark was suitable for mycelium growth.