Effects of different concentrations of Cd^2＋ on the cytosolic Ca^2＋ concentration in Brassica campestris L.pollen were investigated via laser scanning confocal microscopy and the calcium ion-fluorescence indicator fluo-3AM.It is found that the Cytosolic Ca^2＋ distributed polarly in Brassica campestris L.pollen after water culture.The cytosolic Ca^2＋ concentration was higher near the germ furrow and the highest at pollen tube germinating site.The exogenous Cd^2＋ could elevate the intracellular Ca^2＋ concentration,showing that the Brassica campestris L.pollen made the responsion to exoteric stimulative factor by regulating the cytosolic Ca^2＋ concentration.The results provide a new evidence for exploring the toxic mechanism of heavy metal to plant.