The experiment result of effects of paclohutrazol on the growth, yield and quality of barley shows： There is no obvious differences on the period of growth to treatment of growth stages; Effective panicles have a down ward trend with increasing the concentration; And paclobutrazol may reduce the plant height; The low concentration of paclobutrazol increases the number of effective tiller; The grain number of spike of treatments are all higher than that of control group besides the treatment 300mg/kg, and shows the paclobutrazol may increase the grain number of spike. The treatment with different concentrations of paclobutrazol can increase single-spike weight and 1000-grain weight. There is no significant differences between treatments by analysis of variance in yield.